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Updated: Jun 2, 2026
A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
Nineteen novel microsatellite markers for the Olympia oyster, Ostrea conchaphila/lurida
David A Stick1, Chris J Langdon, Michael A Banks
1Department of Fisheries and Wildlife, Coastal Oregon Marine Experiment Station, Oregon State University, USDA-Agricultural Research Service, Hatfield Marine Science Center, 2030 SE Marine Science Drive, Newport, OR 97365, USA.
Abstract:
Accurate evaluation of remnant Ostrea conchaphila/lurida population structure is critical for developing appropriate restoration efforts. Here we report 19 polymorphic microsatellites suitable for analyses of population differentiation, pedigree reconstruction and linkage map construction. We screened clones from four enriched genomic libraries, identified 73 microsatellite-containing sequences and designed polymerase chain reaction primers for 44 of these loci. We successfully optimized polymerase chain reaction conditions for 20 loci, including one monomorphic locus. In a Willapa Bay reference sample, mean observed and expected heterozygosities were 0.6729 and 0.8377. Nine loci deviated from Hardy-Weinberg equilibrium. These markers have proven useful for genetic studies of the Olympia oyster.
