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Isolation of microsatellite loci from expressed sequence tag library of Puccinia striiformis f. sp. tritici
C Q Chen1, W M Zheng, H Buchenauer
1College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China, College of Life Science, Henan Agricultural University, Zhengzhou 450002, China, Institute of Phytomedicine, University Hohenheim, D-70593, Stuttgart, Germany.
Abstract:
We described twenty polymorphic microsatellite loci derived from the expressed sequence tags of Puccinia striiformis f. sp. tritici, which causes yellow rust disease on wheat. The numbers of alleles range from two to six and eight microsatellite loci show significant similarities to known genes. Observed and expected heterozygosities ranged from 0.12 to 0.78 and from 0.24 to 0.87, respectively.
