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Microsatellite markers for the Amazon peacock bass (Cichla piquiti)
D C Carvalho1, D A A Oliveira, I Sampaio
1Molecular Ecology Laboratory, Department of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia; Laboratório de Genética Animal, Departamento de Zootecnia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Laboratório de Genética e Biologia Molecular, Instituto de Estudos Costeiros, Universidade Federal do Pará, Bragança (PA), Brazil.
Abstract:
A set of primers to amplify 10 microsatellite DNA loci was developed for the Neotropical fish Cichla piquiti, one of the largest sized cichlids in the Amazon Basin. These loci were used to genotype individuals from two populations, one native population from the Tocantins River, the other an introduced population in southeast Brazil, Upper Paraná River. Cross-amplification was also successful for another species of peacock bass, C. kelberi. An average of 4.4 alleles per locus (2-9 alleles) was detected. These markers will be useful for the characterization of genetic structure of native populations, and also for invasive biology studies since Cichla species have been introduced in many river basins outside their native ranges.
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