Related Experiment Video
Updated: Jun 2, 2026

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm
Published on: February 5, 2018
Isolation of polymorphic microsatellite markers in the tree sparrow (Passer montanus)
H Izumi1, O Hasegawa, S Higashi
1Graduate School of Environmental Earth Science, Hokkaido University, N10 W5 Kita-ku Sapporo 060-0810, Japan.
Abstract:
To enable the study of the population genetics of the tree sparrow (Passer montanus), which is distributed in many islands of Japan, nine polymorphic microsatellite loci were isolated from 32 individuals. The number of alleles per locus ranged from 2 to 17, and observed heterozygosities ranged from 0.1250 to 0.9375. In eight of the nine loci, the heterozygosities were not significantly different from those expected from Hardy-Weinberg equilibrium. Cross-species amplification in the russet sparrow (P. rutilans) was successful with all primer sets, which were highly polymorphic, suggesting these markers are useful for the population genetics of genus Passer.
Related Concept Videos
Single Nucleotide Polymorphisms-SNPs
Formation of Species

