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Characterization of polymorphic microsatellite markers in the Dalmatian wall lizard Podarcis melisellensis (Squamata:
K Huyghe1, K Breugelmans, M Small
1Department of Biology, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium, Royal Belgian Institute of Natural Sciences, Vautierstraat 29, B-1000 Brussels, Belgium, Molecular Genetics Laboratory, Washington Department of Fish and Wildlife, 600 Capitol Way N., Olympia, WA, 98502, USA, Department of Animal Physiology, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia.
Abstract:
We describe polymerase chain reaction primers and amplification conditions for 13 highly polymorphic microsatellite DNA loci isolated from the Dalmatian wall lizard, Podarcis melisellensis. The number of alleles per locus ranged from 12 to 41, with levels of observed heterozygosity between 0.62 and 0.94. Most of these loci were successfully cross-amplified in the closely related species P. sicula, but levels of polymorphism were always lower.
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