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Published on: October 5, 2017
Isolation and characterization of 11 polymorphic trinucleotide microsatellite markers in the stonefly Arcynopteryx
Kathrin Theissinger1, Kevin A Feldheim, Alfred Seitz
1Department of Limnology and Conservation, Research Institute Senckenberg, Germany Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, Chicago, USA Department of Molecular Ecology, Institute of Zoology, Johannes Gutenberg University, Mainz, Germany.
Abstract:
We describe the isolation of 11 polymorphic trinucleotide microsatellite loci from the stonefly Arcynopteryx compacta. Loci were highly variable with 3 to 14 alleles (mean = 6.45). Observed heterozygosity ranged from 0 to 0.867. Seven loci showed significant deviation from Hardy-Weinberg equilibrium across both populations. There was no evidence for null alleles, and thus, Hardy-Weinberg departures could have resulted from genetic structure between populations or subpopulations. No linkage between loci was found. The 11 loci should prove highly informative for population genetic studies.
