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Published on: October 5, 2017
Development of 10 highly-polymorphic microsatellite markers in the vulnerable Galápagos land iguanas (genus
Sabrina F P Rosa1, Daniel Monteyne, Michel C Milinkovitch
1Laboratory of Evolutionary Genetic, Institute of Molecular Biology and Medicine, Université Libre de Bruxelles, rue Jeener et Brachet 12, 6041 Gosselies, Belgium Laboratory of Natural and Artificial Evolution, Sciences III, Quai Ernest Ansermet 30, 1211 Geneva-4, Switzerland.
Abstract:
The two species of Galápagos land iguanas (Conolophus subcristatus and C. pallidus) are listed as 'vulnerable' species by the International Union for the Conservation of Nature (IUCN Red List; http://www.iucnredlist.org). Here, we report on the isolation and characterization of 10 microsatellite markers using 562 individuals sampled on all Galápagos islands where Conolophus species occur today. We show that these 10 loci are highly polymorphic and display diagnostic alleles for five out of the six island populations. These markers will be useful for Conolophus population genetic analyses as well as for guiding ongoing captive breeding programmes.
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