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Updated: Jun 2, 2026

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
Transferability and characterization of nine microsatellite markers for the tropical tree species Tabebuia roseo-alba
Juliana Massimino Feres1, Marcelo L L Martinez, Carlos A Martinez
1Plant Genetic Laboratory Graduate Program in Genetics, Department of Genetics, FMRP Graduate Program in Comparative Biology, Department of Biology, FFCLRP, University of Sao Paulo, Av. Bandeirantes, 3900, 14040-900, Ribeirao Preto, SP, Brazil.
Abstract:
Microsatellite loci that were previously developed in the tropical tree Tabebuia aurea were used for the genetic analysis of Tabebuia roseo-alba populations. Nine of 10 simple sequence repeat markers were amplified, and the polymorphism was assessed in 58 individuals sampled from two stands in southeastern Brazil. All loci were polymorphic with Mendelian inheritance. The allele numbers were high, ranging from 5 to 13 in population I and 3 to 7 in population II, with means of 8.9 and 5.5, respectively. We conclude that these markers can be efficiently used for parentage and gene-flow studies.
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