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Isolation and characterization of microsatellite markers from the great hornbill, Buceros bicornis
Siriphatr Chamutpong1, Daichi S Saito, Nareerat Viseshakul
1Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand Department of Zoology, National Museum of Nature and Science, Tokyo 169-0073, Japan Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Abstract:
Thirteen polymorphic microsatellite markers were isolated and characterized from the great hornbill, Buceros bicornis. In analyses of 20 individuals, the numbers of alleles per locus varied from two to 11. The expected and observed heterozygosities ranged from 0.22 to 0.88 and from 0.20 to 1.00, respectively. The mean polymorphic information content was 0.62. Among these, three loci deviated from the Hardy-Weinberg equilibrium. However, no significant genotypic disequilibrium was detected between any pair of loci. These microsatellite markers are useful for the population genetic study of the great hornbill.
