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Published on: February 20, 2012
Development and characterization of microsatellite markers for Arenaria grandiflora L. (Caryophyllaceae)
Monika Zavodna1, Lorraine Bottin, Josie Lambourdiere
1Département Ecologie et Gestion de la Biodiversité, CERSP, UMR 5173, Muséum National d'Histoire Naturelle, 61 rue Buffon, 75005 Paris, France, Département Systématique et Evolution, SSM-IFR 101, Muséum National d'Histoire Naturelle, 43 rue Cuvier, 75005 Paris, France.
Abstract:
Genomic libraries of Arenaria grandiflora enriched for di- and trinucleotide repeats were used for the development of novel microsatellite markers. The subset of 13 polymorphic markers was characterized on 40 individuals of A. grandiflora originating from lowland locations in France. The loci amplified 3 to 10 alleles per locus and expected heterozygosities ranged from 0.46 to 0.83. The newly developed markers will be used for population genetic studies and for assessing genetic composition of a restoration experiment of lowland A. grandiflora populations that are protected in France.
