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Updated: Jun 2, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Isolation and characterization of polymorphic microsatellite DNA markers in the sea squirt Halocynthia roretzi
Hyon-Sob Han1, Bo-Hye Nam, Jung-Ha Kang
1Biotechnology Research Center, National Fisheries Research and Development Institute, (NFRDI), Gijang, Busan 619-902, South Korea Aquaculture Environmental Institute, NFRDI, Yongun-ri Sanyang-up, Tongyeong City, Kyeongsangnam-do 650-943, South Korea Department of Microbiology, Pukyong National University, Busan 608-737, South Korea Faculty of Marine Bioscience and Technology, Kangnung National University, Gangneung 210-702, South Korea.
Abstract:
The sea squirt Halocynthia roretzi is an important marine food resource species that is found in the waters around Korea. We describe the isolation and characterization of 13 new polymorphic microsatellite loci in 96 sea squirt samples that were collected from the marine environment of Samcheok on the east coast of Korea. The number of alleles that were observed for each locus ranged from six to 32, and the value of expected and observed heterozygosities was 0.504-0.922 and 0.396-0.813, respectively. These markers will be useful tools for future population studies.
