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Published on: February 20, 2012
Isolation and characterization of polymorphic microsatellite loci from Areca catechu (Arecaceae) using PCR-based
Cheng-Heng Hu1, Chi-Chun Huang, Kuo-Hsiang Hung
1Institute of Anthropology, National Tsing Hua University, Hsinchu, Taiwan 300 Department of Life Sciences, Cheng Kung University, Tainan, Taiwan 701 Taiwan Endemic Species Research Institute, Nantou, Taiwan 552.
Abstract:
Betel nut (Areca nut, Areca catechu L.) is a conspicuous and important cultivated plant of tropical and subtropical habitats throughout Southeast Asia and Oceania. As a significant cultural and social offering, the migration of betel nut associated with human dispersal is an important issue in ethnobotany and anthropology. In this study, we described the development of nine microsatellite loci from A. catechu. The number of alleles per locus ranged from 5 to 15. The expected and observed heterozygosities ranged from 0.71to 0.94 and from 0 to 0.88, respectively. All microsatellite loci, except for AC30, significantly deviated from Hardy-Weinberg equilibrium possibly due to artificially selected cultivation or the existence of excessive null alleles. No linkage disequilibrium was observed from pairwise comparisons of loci, except for AC06 and AC08.
