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Isolation and characterization of 22 polymorphic microsatellite loci for the Barrow's goldeneye (Bucephala islandica)
Sonja Jaari1, Kim Jaatinen, Juha Merilä
1Ecological Genetics Research Unit, Department of Biological and Environmental Sciences, University of Helsinki, PO Box 65, FI-00014 Helsinki, Finland.
Abstract:
We describe primers and polymerase chain reaction conditions to amplify 22 microsatellite loci from the Barrow's goldeneye (Bucephala islandica). The primers were tested on 27 individuals from a population breeding in British Columbia, Canada. The developed primer pairs yielded an average of 6.11 alleles per locus (range 2-12), an average observed heterozygosity of 0.70 (range 0.07-0.96) and a polymorphic information content of 0.07-0.88.
