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Updated: Jun 2, 2026
On-site DNA Detection of Trypanosomatid Parasites and Nosema ceranae Through Alkaline Lysis Coupled to RPA/CRISPR/Cas12a System
Published on: July 18, 2025
Variable microsatellite loci isolated from the Asian honeybee, Apis cerana (Hymenoptera; Apidae)
Jun-Ichi Takahashi1, Souta Shimizu, Satoshi Koyama
1Center of Ecological Research, Kyoto University, Otsu, Shiga 520-2113, Japan, Honeybee Science Research Center, Research Institute, Tamagawa University, Machida, Tokyo 194-8610, Japan.
Abstract:
We developed 12 polymorphic microsatellite loci for the Asian honeybee, Apis cerana using the magnetic particle method. Eight of these 12 were highly polymorphic, having four to seven alleles with an expected heterozygosity of 0.38 to 0.78. The primers also produce polymorphic products in related honeybee species such as Apis nigrocincta. These loci can be used to study parameters associated with genetic structure, such as paternity frequency and worker reproduction.
