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Isolation and characterization of polymorphic microsatellite loci in the green leafhopper Empoasca vitis Goethe
D Papura1, X Giresse, B Chauvin
1UMR Santé Végétale 1065 (INRA-ENITA), ISVV- IFR 103, Centre de Recherches INRA Bordeaux Aquitaine, BP 81, 33883 Villenave d'Ornon cedex, France, UMR BioGeco 1202, Centre de Recherches Forêt Bois INRA Pierroton, 69 route d'Arcachon, 33612 Cestas cedex, France.
Abstract:
Eight dinucleotide microsatellite loci were isolated and characterized within the green leafhopper Empoasca vitis (Goethe) using an enrichment cloning procedure. Primers were tested on 171 individuals collected in the southwest of France from the vine plants. The identified loci were polymorphic, with allelic diversity ranging from two to 18 alleles per locus. Observed heterozygosities were from 0.021 to 0.760. These microsatellite markers should prove to be a useful tool for estimating the population genetic structure, host-plant specialization and migration capacity of this insect.
