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Updated: Jun 2, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Isolation and characterization of microsatellite markers in the Serra Spanish mackerel, Scomberomorus brasiliensis
Mark A Renshaw1, Kory C Douglas, Caird E Rexroad Iii
1Center for Biosystematics and Biodiversity, Department of Wildlife and Fisheries Sciences, Texas A and M University, College Station, TX 77843-2258, USA USDA/ARS National Center for Cold and Cool Water Aquaculture, 11861 Leetown Road, Kearneysville, West Virginia 25430, USA Ministry of Agriculture, Land and Marine Resources, No. 35 Cipriani Boulevard, Newtown, Port of Spain, Trinidad.
Abstract:
Thirteen nuclear-encoded microsatellites from a genomic DNA library of Serra Spanish mackerel, Scomberomorus brasiliensis, were isolated and characterized. The microsatellites include 10 perfect repeats (eight tetranucleotide and two dinucleotide) and three imperfect repeats (two tetranucleotide and one dinucleotide). An additional five microsatellites, isolated originally from two congeneric species (S. cavalla and S. niphonius), were characterized in S. brasiliensis. Serra Spanish mackerel support artisanal fisheries along the Caribbean and Atlantic coasts of Central and South America, from Belize to Brazil.

