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Development of 52 new polymorphic microsatellite markers for the olive flounder, Paralichthys olivaceus
Woo-Jin Kim1, Jung-Ha Kang, Bo-Hye Nam
1Biotechnology Research Institute, National Fisheries Research and Development Institute, Busan 619-705, South Korea Genetics and Breeding Research Center, National Fisheries Research and Development Institute, Geoje 656-842, South Korea Department of Microbiology, Pukyung National University, Busan 608-737, South Korea Department of Molecular Biology, Pusan National University, Busan 609-735, South Korea.
Abstract:
We characterized 52 new microsatellite markers isolated from (GT)(n) and (CT)(n) microsatellite-enriched genomic libraries of the olive flounder (Paralichthys olivaceus). All markers were polymorphic, with eight to 30 (mean 15.1) alleles detected in 30 individuals from a single natural population. Observed heterozygosity ranged from 0.20 to 1.0. Segregation analysis within a mapping family revealed non-amplifying null alleles at six loci. These results indicate that these new microsatellite markers will be useful for population genetic, parentage, and genome mapping studies.
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