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Isolation and characterization of 14 microsatellite loci for the Chevrier's field mouse (Apodemus chevrieri)
Hua Wu1, Xiang-Jiang Zhan, Li Yan
1Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing 100101, China Sichuan Academy of Forestry, Chengdu, Sichuan 610066, China Institute of Rare Animals and Plants, China West Normal University, Nanchong, Sichuan 637002, China.
Abstract:
We describe the isolation and characterization of 14 microsatellite loci in the Chevrier's field mouse from genomic DNA-enriched libraries. The 14 loci were highly polymorphic with numbers of alleles per locus in 24 individuals ranging from three to 13, observed heterozygosity from 0.381 to 0.867 and expected heterozygosity from 0.448 to 0.931. All loci followed Hardy-Weinberg expectations except for CFM3, CFM10 and CFM12 loci. No significant linkage association was found among all these loci. The 14 polymorphic microsatellite loci will be useful in studying phylogeography and population genetics of the Chevrier's field mouse.
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