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Isolation and characterization of microsatellite markers from Robinia pseudoacacia L
Kentaro Mishima1, Tomonori Hirao, Shougo Urano
1Institute of Wood Technology, Akita Prefectural University, 11-1 Kaieisaka, Noshiro, Akita 016-0876, Japan Forestry and Forest Products Research Institute, Forest Tree Breeding Center, 3809-1 Ishi, Juo, Hitachi, Ibaraki 319-1301, Japan.
Abstract:
Microsatellite markers were isolated from Robinia pseudoacacia L. using an enrichment method. Eleven of the 23 primer pairs designed successfully amplified unambiguous and polymorphic single loci among 39 individual R. pseudoacacia L. from northeastern Japan. The observed and expected heterozygosities of the 11 microsatellite markers ranged from 0.333 to 0.821 and from 0.489 to 0.867, respectively. The polymorphisms observed at the 11 microsatellite loci are useful genetic data for forest ecological studies involving R. pseudoacacia L.
