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Updated: Jun 2, 2026
A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
Identification and characterization of 18 novel polymorphic microsatellite makers derived from expressed sequence
C Sauvage1, P Boudry, S Lapègue
1Ifremer, Laboratoire de Génétique et Pathologie, La Tremblade, France.
Abstract:
We report the development of 18 new polymorphic microsatellite DNA markers derived from Crassostrea gigas expressed sequences tags. Genotyping of 48 wild adult oysters sampled from Marennes-Oléron bay (France) revealed 12 to 48 alleles per locus. Observed and expected heterozygosity levels ranged from 0.64 to 1 and from 0.77 to 0.97, respectively. The development of these new markers creates a useful complementary tool for population genetics studies, parentage analysis and mapping in Pacific oyster, a species of major aquacultural and ecological importance.
