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Updated: Jun 2, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Isolation of eight microsatellite markers from Moina macrocopa for assessing cryptic genetic structure in the wild
Haruki Tatsuta1, Izumi Yao, Yoshinari Tanaka
1Research Center for Environmental Risk, National Institute for Environmental Studies, Onogawa, Tsukuba, Ibaraki 305-8506, Japan 21st COE Program (Neoscience of Natural History), Graduate School of Science, Hokkaido University, Kita-ku, Sapporo, Hokkaido 060-0810, Japan.
Abstract:
We isolated eight polymorphic microsatellite loci from the zooplankton Moina macrocopa (Straus), which is sensitive to pollutants such as insecticides and heavy metals. The isolated loci were polymorphic, with three to seven alleles among 23 individuals. Expected heterozygosities ranged from 0.167 to 0.787. These loci can be used to examine cryptic genetic structure and to infer the connectivity among metapopulations.
