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Isolation and characterization of microsatellite loci for storm-petrels
Zhengxin Sun1, Elena Gómez-Díaz, Anna Bailie
1Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6 Departament Biologia Animal, Universitat de Barcelona, Avenue Diagonal 645, 08028 Barcelona, Spain.
Abstract:
Primers were developed for 10 microsatellite loci for two species of Oceanodroma storm-petrels. Variability was tested in 27 O. castro and 22 O. monteiroi from the Azores, and 24 O. leucorhoa from Norway. At least six loci amplified reliably and were polymorphic in each species. The number of alleles per locus averaged 4.6, and observed heterozygosities averaged 0.41. Most primers also yielded polymerase chain reaction products in O. tethys, O. hornbyi and Pterodroma phaeopygia. These loci are being used to assay population genetic structure in storm-petrels.
