Isolation of 11 microsatellite markers in Crepidula convexa (Gastropoda, Calyptraeideae) for parentage analyses
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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Claire Daguin-Thiébaut1, Sabrina LE Cam, Frédérique Viard
1UPMC Univ Paris 06, UMR 7144, Adaptation & Diversité en Milieu Marin, Station Biologique de Roscoff, 29682 Roscoff, France CNRS, UMR 7144, Station Biologique de Roscoff, 29682 Roscoff, France.
Abstract:
Crepidula convexa, a calyptreid gastropod with direct embryonic development, changes sex from male to female in the course of its lifetime (protandry). Under sex-allocation theory, male reproductive success should be independent from age and size (a proxy used for age). However, this may be counterbalanced by female cryptic choice or gregarious behaviour. Eleven polymorphic microsatellite loci were thus developed to examine paternity of embryos and larvae. This set of loci appears suitable to carry out paternity analyses due to the high exclusion probability of unrelated males given the maternal genotype.

