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Published on: October 29, 2018
Isolation and characterization of microsatellite loci for the Asian longhorned beetle, Anoplophora glabripennis
Maureen Carter1, Alexandra M Casa, Mahmoud Zeid
1Department of Entomology, Comstock Hall, Department of Plant Breeding, Emerson Hall Institute for Genomic Diversity, Biotechnology Building, Cornell University, Ithaca, NY 14853, USA.
Abstract:
We describe the first isolation of SSR (simple sequence repeat) markers for Anoplophora glabripennis, a pest of forests in China and urban trees in the USA. Ten markers were developed using FIASCO (fast isolation by AFLP of sequences containing repeats) with an (AC)(17) probe, and five markers were identified in expressed sequence tags found on the NCBI database. Twenty-seven beetles from a population in Gansu Province, China, were assayed. The number of alleles ranged from 3 to 9; observed and expected heterozygosities ranged from 0.07 to 0.79 and from 0.30 to 0.78, respectively.
