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Published on: May 27, 2022
Microsatellite markers for the diploid basidiomycete fungus Armillaria mellea
K Baumgartner1, L C Grubisha, P Fujiyoshi
1USDA-ARS, 363 Hutchison Hall, University of California, One Shields Avenue, Davis, CA 95616, USA, USDA-ARS, Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA, Department of Environmental Science, Policy and Management, Division of Ecosystem Science, University of California, Berkeley, Berkeley, CA 94720, USA, Biology Department, Middle Tennessee State University, PO Box 60, Murfreesboro, TN 37132, USA.
Abstract:
We isolated and characterized 12 microsatellite markers for two North American populations (California, Pennsylvania) of Armillaria mellea, a fungal pathogen responsible for Armillaria root disease of numerous woody plants. Allele frequency ranged from two to nine alleles per locus, and gene diversity ranged from 0.05 to 0.86. Of the 12 loci, eight loci were polymorphic in the California and Pennsylvania populations, and showed no evidence of heterozygote deficiencies or severe linkage disequilibrium. Our results suggest that we have isolated and characterized variable loci to estimate genotypic diversity, gene flow and migration, and to determine population structure of North American A. mellea.
