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Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
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Development of microsatellite markers for blunt snout bream Megalobrama amblycephala using 5'-anchored PCR

Shou-Jie Tang1, Si-Fa Li, Wan-Qi Cai

  • 1Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem, Ministry of Agriculture, Shanghai Ocean University, 999 Hu Cheng Ring Road, Shanghai 201306, China.

Molecular Ecology Resources
|May 14, 2011
PubMed

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Southern Blot02:57

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...