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Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
Published on: May 27, 2022
Microsatellite markers for the ectomycorrhizal basidiomycete Lactarius mammosus
N Högberg1, A Guidot, M Jonsson
1Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, PO Box 7026, S-750 07 Uppsala, Sweden, Laboratoire d'Ecologie Microbienne (UMR CNRS 5557), Université Claude Bernard Lyon 1, Bât. A. Lwoff, 43 Bd. du 11 Novembre 1918, F-69622 Villeurbanne Cedex, France.
Abstract:
We report the characterization of polymorphic microsatellite markers in the ectomycorrhizal fungus Lactarius mammosus Fr. Two enrichment protocols were used to isolate microsatellite loci and polymorphism was explored within 31 sporocarps originating from a forest site in northern Sweden. We found nine variable microsatellite loci with the number of alleles per locus varying between 2 and 5, and expected heterozygosities ranging from 0 to 0.84. These loci are available for the analysis of genetic structure and gene flow in L. mammosus populations.

