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Updated: Jun 2, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Isolation and characterization of new microsatellite markers for rose bitterlings, Rhodeus ocellatus
1Kagawa Prefectural Research Institute for Environmental Sciences and Public Health, 5-3-105 Asahimachi, Takamatsu, 760-0065, Japan Gene Research Center, Kagawa University, 2393, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0795, Japan Department of Life Sciences, Kagawa University, 2393, Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0795, Japan.
Abstract:
The Japanese rose bitterling (Rhodeus ocellatus kurumeus) is facing imminent extinction because of hybridization and competition from an invasive alien subspecies (Rhodeus ocellatus ocellatus). Eleven new microsatellite markers for the two subspecies were developed using dinucleotide repeat specific polymerase chain reaction. The number of alleles per locus and the heterozygosity in R. o. kurumeus were lower than those in R. o. ocellatus. Most of these microsatellite markers were successfully cross-amplified in three Acheilognathinae species.
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