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Published on: March 13, 2011
Polymorphic microsatellite markers for the critically endangered Balearic shearwater, Puffinus mauretanicus
E G Gonzalez1, M Genovart, D Oro
1Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales, CSIC, José Gutiérrez Abascal 2, 28006 Madrid, Spain, Department of Biochemistry and Molecular Biology IV, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain, Instituto Mediterráneo de Estudios Avanzados IMEDEA (CSIC-UIB), Miquel Marquès 21, 07190 Esporles, Mallorca, Spain, Estación Biológica de Doñana (CSIC), Avda. Mª Luisa s/n, 41080 Sevilla, Spain.
Abstract:
Ten novel polymorphic microsatellite loci were isolated and characterized from the Balearic shearwater (Puffinus mauretanicus), a critically endangered seabird. The developed loci revealed a relatively low number of alleles per locus, as well as low levels of polymorphism (H(O) = 0.377 ± 0.241). One of the loci appeared to be W-linked. All polymorphic loci were successfully amplified in its closely related species, the Yelkouan shearwater (Puffinus yelkouan). These microsatellite markers would be useful for assessing population structure in the Balearic shearwater and the possible hybridization process between both shearwaters species.
