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Published on: February 20, 2012
Isolation and characterization of 11 new microsatellite markers for Macaranga (Euphorbiaceae)
Christina Baier1, Daniela Guicking, Kathleen Prinz
1Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany, Plant Molecular Systematics, University of Kassel, 34109 Kassel, Germany, Institute for Plant Genetics, University of Hannover, 30419 Hannover, Germany.
Abstract:
We provide primer sequences for 11 new polymorphic microsatellite markers developed in the tropical ant-plant genus Macaranga (Euphorbiaceae), after enrichment cloning of Macaranga tanarius and Macaranga hypoleuca. Allele numbers per locus ranged from two to 16 among 20 accessions of M. tanarius, and from three to 10 among 22 accessions of M. hypoleuca. Observed and expected heterozygosities ranged from 0.150 to 0.900 and from 0.375 to 0.894 in M. tanarius, and from 0.545 to 1.000 and from 0.434 to 0.870 in M. hypoleuca, respectively. Six of the 11 primer pairs successfully cross-amplified polymorphic polymerase chain reaction products in Macaranga winkleri.
