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Updated: Jun 2, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
New polymorphic microsatellite markers and development of mitotyping primers for West Mediterranean green toad
Caroline Colliard1, Alessandra Sicilia, Craig Moritz
1Department of Ecology and Evolution, Biophore, University of Lausanne, CH-1015 Lausanne, Switzerland.
Abstract:
We report new polymorphic microsatellites for three species of Palearctic green toads (Bufo viridis subgroup): 10 in B. balearicus and seven each in B. siculus and B. boulengeri. Diversity at these loci, measured for 27 B. balearicus, 23 B. siculus and 11 B. boulengeri, ranged from low to high (two to 10 alleles). Mitotyping primers, specific to the control region, which allow fast screening of parapatric Sicilian endemic B. siculus and Italian mainland-origin B. balearicus, were developed.

