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Updated: Jun 2, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Polymerase chain reaction primers for polymorphic microsatellite loci in the ant Pseudomyrmex gracilis (Formicidae:
Volker S Schmid1, Stefanie Kautz, Andreas Trindl
1Biologie I, Universität Regensburg, D-93040 Regensburg, Germany. volker.schmid@biologie.uni-regensburg.de
Abstract:
The elongate twig ant Pseudomyrmex gracilis is widely distributed in the New World ranging from southern USA to Brazil and northern Argentina. To investigate its within-colony relatedness, we developed primers for nine polymorphic microsatellite loci. High levels of within-population variation were observed, with number of alleles ranging from two to 13, and heterozygosity from 0.28 to 0.90. The loci showed no deviations from Hardy-Weinberg equilibrium and no linkage disequilibrium.

