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Published on: March 13, 2011
Isolation of 21 polymorphic microsatellite markers for the Virginia opossum (Didelphis virginiana)
Jennifer A Fike1, James C Beasley, Olin E Rhodes
1Department of Forestry and Natural Resources, Purdue University, 715 West State Street, West Lafayette, IN 47907, USA. jfike@purdue.edu
Abstract:
Twenty-one polymorphic microsatellite markers were developed for the Virginia opossum (Didelphis virginiana). The number of alleles ranged from two to 13 and observed heterozygosities ranged from 0.464 to 0.964. Significant heterozygote deficiencies were observed at three loci and null alleles were detected at five loci. Evidence for gametic disequilibrium was observed between three sets of paired loci after a sequential Bonferroni correction was applied. These markers will enable us to investigate the mating tactics, movement behaviour and social structure of Virginia opossum populations inhabiting fragmented agricultural landscapes.
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