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Updated: Jun 2, 2026
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Characterization of polymorphic microsatellite DNA markers in Hakea oldfieldii Benth. (Proteaceae)
1Science Division, Department of Environment and Conservation, Locked Bag 104, Bentley Delivery Centre, Bentley, WA 6983, Australia. margaret.byrne@dec.wa.gov.au
Abstract:
A genomic library was constructed and 13 polymorphic microsatellite markers were developed for Hakea oldfieldii, a woody shrub endemic to southwest Western Australia. Polymorphism was investigated for these markers in 28 individuals from a single population located in restricted habitat at the base of the Whicher Range south of Busselton. Expected heterozygosity ranged from 0.279 to 0.770 and averaged 0.633. Observed heterozygosity ranged from 0.321 to 0.786 and averaged 0.598. The number of alleles per locus ranged from 2.0 to 6.0 and averaged 4.5. These markers will be used to assay genetic diversity and pollen dispersal in this species.
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