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Published on: October 5, 2017
Characterization of polymorphic microsatellite DNA markers in the black-tailed godwit (Limosa limosa: Aves)
Yvonne I Verkuil1, Krijn Trimbos, Oliver Haddrath
1Animal Ecology Group, Centre of Ecological and Evolutionary Studies (CEES), University of Groningen, PO Box 14, 9750 AA Haren, The Netherlands.
Abstract:
We isolated and tested 16 microsatellite loci in black-tailed godwits from the Netherlands (Limosa limosa limosa), and from Australasia (subspecies melanuroides). One locus was monomorphic, two loci had null-alleles and one was significantly heterozygote deficient. The remaining 12 polymorphic loci had on average 7.9 alleles (range 5-11) and the mean expected heterozygosity was 0.69. No significant linkage disequilibrium between the loci was observed and all loci were autosomal. Fourteen loci were successfully cross-amplified in bar-tailed godwit (Limosa lapponica).
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