Microsatellite markers for Spergularia media (L.) C. Presl. (Caryophyllaceae) and their cross-species transferability
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Published on: February 20, 2012
Kathleen Prinz1, Stephan Schie, Thomas Debener
1Plant Molecular Systematics, University of Kassel, 34109 Kassel, Germany Institute of Biology/Geobotany and Botanical Garden, Martin-Luther-University, Halle-Wittenberg, Germany Institute for Plant Genetics, University of Hannover, Hannover, Germany.
Abstract:
We developed 11 polymorphic microsatellite markers for Spergularia media (Caryophyllaceae), a perennial halophyte of coastal salt meadows and continental areas of western Eurasia. The number of alleles per locus observed in a single population of 20 individuals from the German North Sea coast ranged from 3 to 20. Observed and expected heterozygosities ranged from 0.200 to 0.850 and from 0.278 to 0.936 respectively. Observed heterozygosities were lower than expected heterozygosities at all loci, presumably as a consequence of inbreeding. All markers cross-amplified in the closely related S. salina. Of these, nine were polymorphic in the heterologous species. Only two primer pairs generated PCR products in S. diandra.
