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Published on: October 5, 2017
Isolation and evaluation of 18 microsatellite markers in Siniperca chuatsi (Basilewsky)
Gangqiao Kuang1, Shuangqing Lu, Shuming Zheng
1Department of Fisheries, Rongchang Campus, Southwest University, Rongchang, Chongqing 402460, China Department of Biotechnology and Environment Science, Changsha University, Changsha 410003, China.
Abstract:
Eighteen novel microsatellite markers were isolated in the Chinese perch Siniperca chuatsi (Basilewsky) using the FIASCO method, from (AC/TG)(n) , (AG/TC)(n) , (AT/TA)(n) , (GATA/CTAT)(n) and (GATT/CTAA)(n) repeat genomic libraries. The number of alleles per locus ranged from two to eight in a sample of 30 individuals from a wild population. Observed heterozygosity was between 0.100 and 0.737. Seven loci showed significant deviation from Hardy-Weinberg equilibrium, null alleles were suggested at nine loci but no linkage disequilibrium between loci was detected. These loci could be useful in the population genetic study of S. chuatsi.
