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Updated: Jun 2, 2026
In situ Protocol for Butterfly Pupal Wings Using Riboprobes
Published on: May 28, 2007
Microsatellite markers associated with genes expressed in developing wings of Bicyclus anynana butterflies
Patrícia Beldade1, Arjèn E Van't Hof, Maria Adelina Jerónimo
1Institute of Biology Leiden University, PO Box 9516, 2300 RA, Leiden, The Netherlands Instituto Gulbenkian de Ciência, Apartado 14, P-2781-901 Oeiras, Portugal School of Biological Sciences, Biosciences Building, Crown Street, University of Liverpool, Liverpool L69 7ZB, UK Department of Ecology and Evolutionary Biology, University of California at Irvine, 5210 McGaugh Hall, Irvine, 92697-2525 CA, USA.
Abstract:
Deriving useful microsatellite markers in lepidopterans has been challenging when relying on scans of genomic DNA libraries, presumably due to repetitiveness in their genomes. We assayed 96 of 320 microsatellites identified in silico from a collection of Bicyclus anynana ESTs, in 11 independent individuals from a laboratory population. From the 68 successful assays, we identified 40 polymorphic markers including 22 with BLAST-based annotation. Nine of 12 selected polymorphic markers tested in a panel of 24 wild-caught individuals converted to successful assays and were all polymorphic. We discuss how microsatellite discovery in ESTs is an efficient strategy with important attendant advantages.
