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Updated: Jun 2, 2026

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Ten novel microsatellite loci characterized for a remarkably widespread fish: Galaxias maculatus (Galaxiidae)
Cecilia Carrea1, Ian G Paterson, Victor E Cussac
1INIBIOMA (Universidad Nacional del Comahue-CONICET) Bariloche, 8400 Río Negro, Argentina Department of Biology, Dalhousie University, Halifax, NS, Canada B3H 4J1.
Abstract:
Ten polymorphic microsatellite markers (five tetra-, one compound tetra-, one octa- and three dinucleotides) were isolated and characterized for Galaxias maculatus, a fish species widely distributed in the Southern Hemisphere. Markers were tested in 89 individual samples from a single location and the number of alleles ranged between 2 and 28. Observed and expected heterozygosities ranged from 0.103 to 0.910 and 0.098 to 0.935 respectively. No evidence was detected for either linkage disequilibrium (P-values > 0.05 for each locus pair) or deviations from HWE (P-values > 0.05 for every loci).
