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In Vitro Rearing of Solitary Bees: A Tool for Assessing Larval Risk Factors
Published on: July 16, 2018
Characterization of 12 polymorphic microsatellite markers for a facultatively eusocial sweat bee (Megalopta genalis)
Karen M Kapheim1, John P Pollinger, William T Wcislo
1Department of Ecology and Evolutionary Biology, 621 Charles E. Young Dr. South, University of California, Los Angeles, CA 90095 USA Smithsonian Tropical Research Institute, Box 0843-03092, Balboa, Ancon, Republic of Panama.
Abstract:
We developed a library of twelve polymorphic di- and tri-nucleotide microsatellite markers for Megalopta genalis, a facultatively eusocial sweat bee. We tested each locus in a panel of 23 unrelated females and found 7-20 alleles per locus. Observed and expected heterozygosities ranged from 0.65 to 0.96 and from 0.69 to 0.95 respectively. None of the loci deviated from Hardy-Weinberg equilibrium proportions or was found to be in gametic disequilibrium.
