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A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
Published on: March 13, 2011
Microsatellite markers for the Common ringtail possum (Pseudocheirus peregrinus) and their amplification in other
Melanie L Lancaster1, Steven J B Cooper, Sue Carthew
1School of Earth and Environmental Science, The University of Adelaide, Adelaide, SA 5005, Australia Evolutionary Biology Unit, South Australian Museum, Adelaide, SA 5000, Australia Australian Centre for Evolutionary Biology and Biodiversity, The University of Adelaide, Adelaide, SA 5005, Australia Biological Sciences, Monash University, Clayton, Vic., 3800, Australia.
Abstract:
Eleven microsatellite markers were developed for the Common ringtail possum, an arboreal marsupial abundant in fragmented forests of south-eastern Australia. Loci were highly polymorphic (4-32 alleles per locus) and heterozygosity ranged from 0.66 to 1. Two loci deviated significantly from Hardy-Weinberg equilibrium proportions, possibly because of low-frequency null alleles. These markers will be informative for examining patterns of gene flow, relatedness and mating systems within fragmented populations of the Common ringtail possum and have potential for use in other Pseudocheirids.
