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Development of polymorphic microsatellite markers for the dung fly (Sepsis cynipsea)
Maja P Greminger1, Martin A Schäfer, Alexander Nater
1Anthropological Institute & Museum, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland Zoological Museum, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
Abstract:
The polyandrous fly Sepsis cynipsea has been used extensively in studies of sexual selection and local adaptation. We isolated and characterized 11 novel microsatellite markers for S. cynipsea from a genomic library and screened 32 flies for polymorphism. All microsatellite markers show high allelic diversity with an average of 9.64 alleles per locus. Two microsatellites were found likely to be X-linked. These novel markers will significantly advance studies of sexual selection and evolutionary genetics of S. cynipsea and related species, especially given the low numbers of markers currently available in this family.
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