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Isolation and characterization of 10 polymorphic microsatellite markers from striped marlin, Tetrapturus audax
C M Purcell1, J S Harrison, S Edmands
1Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0371, USA Department of Biology, Georgia Southern University, Statesboro, GA 30460, USA.
Abstract:
We present the isolation and characterization of 10 microsatellite loci for striped marlin, Tetrapturus audax. Thirty individuals from each of four locations revealed that all loci were polymorphic with two to 31 alleles per locus. Observed levels of heterozygosity ranged from 0.3000 to 0.9667. Significant deviations from Hardy-Weinberg equilibrium were detected in two loci, TA105 in Hawaii and New Zealand and TA155 in Hawaii, and null alleles may be present in loci TA105 and TA155 in those locations, and in locus TA193 in Mexico. No significant linkage disequilibrium was detected in any pairwise-locus comparison.
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