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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jun 2, 2026

Extraction of Diatom DNA from Water Samples and Tissues

Extraction of Diatom DNA from Water Samples and Tissues

Published on: November 10, 2023

Barcoding diatoms: Is there a good marker?

Mónica B J Moniz¹, Irena Kaczmarska

¹Biology Department, Mount Allison University, 63B York Street, Sackville, NB, Canada E4L lG7.

Molecular Ecology Resources

|May 14, 2011

Summary

This summary is machine-generated.

The 5.8S + ITS-2 gene region is the best DNA barcode for diatoms, offering high variability and successful amplification for species identification. This marker aids in distinguishing diatom species effectively.

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Area of Science:

Microbiology
Molecular Biology
Ecology

Background:

DNA barcoding utilizes small DNA fragment divergence to identify biological species.
Diatoms, crucial primary producers, require reliable identification methods for ecological studies.

Purpose of the Study:

To evaluate three genetic markers for their efficacy as DNA barcodes in diatoms.
To identify the most suitable marker for accurate diatom species discrimination.

Main Methods:

Analysis of 44 sequences per marker (small ribosomal subunit, cytochrome c oxidase subunit 1, 5.8S + ITS-2) from 28 diatom species.
Calculation of sequence alignment and uncorrected genetic distances (P) at intra- and heterospecific levels.

Main Results:

All three markers (small ribosomal subunit, cytochrome c oxidase subunit 1, 5.8S + ITS-2) successfully separated diatom species.
5.8S + ITS-2 showed high amplification/sequencing success and variability, though alignment required secondary structure consideration.
Cytochrome c oxidase subunit 1 had high divergence but low amplification success; small ribosomal subunit had high data availability but required longer fragments.

Conclusions:

The 5.8S + ITS-2 fragment is proposed as the optimal DNA barcode for diatoms due to its variability and amplification success.
This marker's potential to indicate sexual compatibility offers an added advantage for diatom research.