Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trained nnU-Net model for semantic segmentation of human adult cervical vertebrae from CT-Scans.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

Na<sub>2</sub>VO(HPO<sub>4</sub>)<sub>2</sub>: an original phase solved by continuous 3D electron diffraction and powder X-ray diffraction.

Dalton transactions (Cambridge, England : 2003)·2021
Same author

Introducing article numbering to JournaldeMycologieMédicale.

Journal de mycologie medicale·2020
Same author

[Feasibility and benefits of training to screen for chronic obstructive pulmonary disease].

Revue des maladies respiratoires·2019
Same author

[Lower extremity arteriopathy disease in the elderly: A systematic review].

Annales de cardiologie et d'angeiologie·2018
Same author

Performance of semiconductor sequencing platform for non-invasive prenatal genetic screening for fetal aneuploidy: results from a multicenter prospective cohort study in a clinical setting.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2018
Same journal

From Gene Copies to Cell Numbers: Advancing Quantitative Approaches in Protistan Ecology Using Digital PCR.

Molecular ecology resources·2026
Same journal

EasyCen: A Lightweight Framework for Centromere Localisation and Repeat-Organisation Profiling in Telomere-to-Telomere Genomes.

Molecular ecology resources·2026
Same journal

A Practical Framework for GT-Seq Panel Optimization.

Molecular ecology resources·2026
Same journal

Comparison of Environmental DNA and Bulk DNA Metabarcoding for Assessing Terrestrial Arthropod Diversity Across Three Habitat Types on Guam.

Molecular ecology resources·2026
Same journal

pr2-Wormifier: A Bioinformatics Pipeline to Create Custom Reference Databases for Improved Metabarcoding of Marine Protists.

Molecular ecology resources·2026
Same journal

Individual Identification of Prey in Carnivore Scats.

Molecular ecology resources·2026
See all related articles

Related Experiment Video

Updated: Jun 2, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Current trends in microsatellite genotyping.

E Guichoux1, L Lagache, S Wagner

  • 1INRA, UMR 1202 Biodiversity Genes & Communities, F-33610 Cestas, France.

Molecular Ecology Resources
|May 14, 2011
PubMed
Summary
This summary is machine-generated.

Microsatellite (SSR) markers remain valuable for population genetics despite new technologies. Current studies often miss quality controls and multiplex PCR, hindering efficient use of advanced techniques.

More Related Videos

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Related Experiment Videos

Last Updated: Jun 2, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Area of Science:

  • Genetics
  • Molecular Biology
  • Population Genetics

Background:

  • Microsatellites (simple sequence repeats) have been widely used as molecular markers since the late 1980s.
  • Despite advancements in genotyping and sequencing, microsatellite marker utility is increasing due to technical improvements.
  • Multiplex PCR and next-generation sequencing (NGS) offer cost-effective, high-throughput methods for microsatellite analysis.

Purpose of the Study:

  • To evaluate current practices in population genetic studies using microsatellite markers.
  • To highlight the gap between available technological advancements and their adoption in research.
  • To advocate for standardized protocols and bioinformatic tools for microsatellite analysis.

Main Methods:

  • Survey of recently published population genetic studies utilizing simple sequence repeats.
  • Analysis of the adoption rates of quality controls and multiplex PCR techniques.
  • Review of technical advances in microsatellite genotyping and sequencing.

Main Results:

  • Over 50% of recent population genetic studies using microsatellites lack essential quality controls.
  • A significant portion of studies do not implement multiplex PCR, a cost- and time-saving technique.
  • Despite improvements in NGS and multiplexing, current research practices lag behind technological potential.

Conclusions:

  • There is a critical need to update current practices in microsatellite marker application.
  • Implementing standardized high-throughput protocols and bioinformatic tools is essential for efficient microsatellite analysis.
  • Adopting advanced techniques like multiplex PCR and leveraging NGS data will enhance the quality and efficiency of population genetic studies.