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Cell-mediated Immune Responses

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Related Experiment Video

Updated: Jun 2, 2026

Isolation and Transplantation of Different Aged Murine Thymic Grafts.

Isolation and Transplantation of Different Aged Murine Thymic Grafts.

Published on: May 13, 2015

Thymic microenvironment reconstitution after postnatal human thymus transplantation.

Bin Li¹, Jie Li, Blythe H Devlin

¹Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA.

Clinical Immunology (Orlando, Fla.)

|May 14, 2011

Summary

Cultured thymus transplants restore function in DiGeorge anomaly. Thymic epithelial cells initially express both cortical and medullary markers, then differentiate to form distinct thymic structures.

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Last Updated: Jun 2, 2026

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Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Published on: January 7, 2012

Area of Science:

Immunology
Developmental Biology
Cell Biology

Background:

Complete DiGeorge anomaly results in a non-functional thymus.
Thymus transplantation can restore immune function in affected individuals.
The cellular mechanisms driving thymus regeneration remain incompletely understood.

Purpose of the Study:

To investigate the cellular and molecular processes underlying thymus regeneration after transplantation in DiGeorge anomaly.
To characterize the differentiation pathway of thymic epithelial cells in developing neo-thymus.

Main Methods:

Analysis of 7 post-transplantation thymus biopsy tissues.
Immunohistochemical evaluation of thymocyte and epithelial cell markers, including cortical dendritic reticulum antigen 2 (CDR2) and cytokeratin (CK) 14.
Assessment of epithelial cell adhesion molecule (EpCAM) expression.

Main Results:

Biopsies showed varying stages of thymic development, from predominantly cortical structures to distinct mature cortex and medulla.
Thymic epithelium unexpectedly expressed both cortical (CDR2) and medullary (CK14) markers in early stages.
Epithelial cell adhesion molecule (EpCAM) indicated early medullary development.
Mature tissues exhibited normal compartmentalization of CDR2 and CK14.

Conclusions:

Thymic epithelial cells possess progenitor characteristics, expressing both cortical and medullary markers.
Post-transplantation, these progenitor cells proliferate and associate with thymocytes.
Subsequent differentiation leads to the formation of distinct cortical and medullary epithelial compartments, restoring thymic architecture.