Differential expression of PRLIPs, a pathogenesis-related gene family encoding class 3 lipase-like proteins in...

In plants plenty of inducible defense-related proteins classified into 17 pathogenesis-related (PR) families have been described. Expression of homologous PR genes from the same family can be induced by the different defense hormones, like salicylic acid (SA), jasmonic acid (JA) or ethylene (ET), and are also regulated in a organ- or tissue-specific manner. A recently identified pathogenesis-related gene family, the PRLIP (pathogenesis-related lipase) has 9 members in Arabidopsis and their organization and expression pattern - as it is summarized in this study - is similar to the one of genes coding for other PR proteins. PRLIP3, PRLIP8 and PRLIP9 showed a relatively high expression in all tissues tested with a maximum in root (PRLIP3), stem (PRLIP8) or siliques (PRLIP9). The activity of PRLIP3 gene was further induced by SA and JA treatment. Other members (PRLIP1, PRLIP2, PRLIP4 and PRLIP6), however, were detected only in some of the tested organs. High levels of PRLIP1 mRNA occurred in all green tissues and in siliques, while in the latter PRLIP2 also displayed high expression. PRLIP6 and PRLIP4 exhibited root specific transcription while no mRNAs of PRLIP5 and PRLIP7 were detected in any plant tissues examined. In leaves SA treatment enhanced PRLIP1 and PRLIP2 expression, JA treatment induced PRLIP6 and ET treatment upregulated both PRLIP1 and PRLIP6. This organization and expression diversity of the PRLIP gene family is typical to plant PR genes suggesting the encoded proteins might serve essential functions in plant defense or priming.