Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein phosphorylation in developing and regenerating rat kidney.

T Hoang1, M Bergeron

  • 1Département de Physiologie, Université de Montréal, Québec, Canada.

Cell and Tissue Kinetics
|March 1, 1990
PubMed
Summary

Protein phosphorylation in rat kidneys changes significantly during neonatal development but not compensatory growth. This suggests hormonal regulation plays a key role in kidney development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SPINAL CANAL SIZE IMPROVEMENT AFTER XLIF FOR LUMBAR SPINAL STENOSIS.

Georgian medical news·2025
Same author

Correlations between the pediatric sleep questionnaire and the pediatric overnight pulse oximetry.

International journal of pediatric otorhinolaryngology·2025
Same author

Soft wetting with (a)symmetric Shuttleworth effect.

Proceedings. Mathematical, physical, and engineering sciences·2022
Same author

Odderon Exchange from Elastic Scattering Differences between pp and pp[over ¯] Data at 1.96 TeV and from pp Forward Scattering Measurements.

Physical review letters·2021
Same author

EBV-associated primary CNS lymphoma occurring after immunosuppression is a distinct immunobiological entity.

Blood·2020
Same author

[Roles and impacts of pharmaceutical activity from 1990 to today: Literature review and research perspectives].

Annales pharmaceutiques francaises·2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Developmental Biology

Background:

  • Protein phosphorylation is a critical regulatory mechanism in cellular processes.
  • Kidney development and growth are complex processes influenced by various factors.
  • Cyclic adenosine monophosphate-dependent protein kinase (cAMP-PK) is involved in cellular signaling pathways.

Purpose of the Study:

  • To investigate age-dependent changes in protein phosphorylation in rat renal cytosolic extracts.
  • To examine the impact of mononephrectomy on renal protein phosphorylation.
  • To correlate changes in protein phosphorylation with the activity of cAMP-dependent protein kinase during kidney development and compensatory growth.

Main Methods:

  • Preparation of rat renal cytosolic extracts from different age groups and mononephrectomized rats.
  • Incubation with gamma-[32P]ATP to label phosphorylated proteins.
  • Analysis using high-resolution two-dimensional electrophoresis and autoradiography.
  • Assay of cAMP-dependent protein kinase activity and [3H]cAMP binding.

Main Results:

  • Significant differences in the pattern and intensity of protein phosphorylation were observed between neonatal and mature rats.
  • Neonatal rat kidney extracts showed prominent phosphorylation of proteins between the origin and Mr 52,000, including a group near phosphorylase b (Mr 97,000).
  • Mature rat kidney extracts exhibited intense phosphorylation of lower molecular weight proteins (Mr 60,000–44,000).
  • Mononephrectomy did not alter the number or intensity of phosphorylated proteins compared to normal rats.
  • cAMP-PK activity and cAMP binding were modified during neonatal development but not during compensatory renal growth.

Conclusions:

  • Renal protein phosphorylation patterns undergo substantial changes during neonatal development, indicating dynamic regulatory processes.
  • Compensatory renal growth following mononephrectomy does not significantly alter protein phosphorylation.
  • The observed developmental changes in protein phosphorylation and cAMP-PK activity suggest a role for hormonal signaling in kidney growth and development.

Related Experiment Videos