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Updated: Jun 2, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
Isolation of a bacterium possessing a haloacid dehalogenase from a marine sediment core
Yoko Chiba1, Takashi Yoshida, Norihiro Ito
1Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University.
Abstract:
A monobromoacetic acid-resistant bacterium, Bacillus strain I37c, was isolated from a marine sediment core. The strain grew in a medium containing 1.8 mg mL(-1) of monobromoacetic acid. It produced constitutively a 2-haloacid dehalogenase that catalyzed the dehalogenation of monobromoacetic acid, monochloroacetic acid, and both L- and D-2-chloropropionic acid. The optimal pH and temperature for the activity measured using a partly purified enzyme were similar to those of known group I haloacid dehalogenases.
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