Related Experiment Video
Updated: Jun 2, 2026
14:57
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
Tissue-specific apoptotic effects of the p53 codon 72 polymorphism in a mouse model
Gregory A Azzam1, Amanda K Frank, Monica Hollstein
1Fox Chase Cancer Center; Philadelphia PA, USA.
Cell Cycle (Georgetown, Tex.)
|May 14, 2011
Summary
Mouse models accurately reflect human cancer risk associated with genetic variations. A specific p53 polymorphism
Area of Science:
- Genetics
- Cancer Biology
- Molecular Biology
Background:
- Human cancer risk is influenced by numerous genetic polymorphisms, but association studies face challenges like genetic heterogeneity and limited sample sizes.
- Mouse models offer a solution by replicating human variants to clarify their role in cancer susceptibility.
Purpose of the Study:
- To investigate the impact of the codon 72 polymorphism in p53 on cancer risk and apoptotic function.
- To demonstrate the utility of mouse models in studying human cancer susceptibility variants.
Main Methods:
- Development of a mouse model for the human codon 72 p53 polymorphism.
- Analysis of the polymorphism's effect on p53's apoptotic function and cooperation with NF-kB.
Main Results:
- The codon 72 p53 polymorphism was found to regulate apoptosis by influencing cooperation with NF-kB.
- This polymorphism exhibits tissue-specific effects on apoptotic function, adding complexity to human cancer risk assessment.
Conclusions:
- Mouse models effectively replicate human cancer phenotypes associated with specific polymorphisms.
- Modeling human variants in mice is a powerful approach to assess their impact on cancer risk, progression, and therapeutic responses.
More Related Videos
04:56Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
Published on: December 30, 2025
10:13Modeling Astrocytoma Pathogenesis In Vitro and In Vivo Using Cortical Astrocytes or Neural Stem Cells from Conditional, Genetically Engineered Mice
Published on: August 12, 2014
Related Concept Videos
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
02:36DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:36DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
01:31The Intrinsic Apoptotic Pathway
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...